MALDI Target Spotting

Fast, automated protein characterization is critical for large-scale proteomic research. For high throughput proteomics and target identification, complex protein mixtures are separated by two-dimensional electrophoresis and the resulting protein spots are analyzed by mass spectrometry. Mass spectrometry can provide total protein mass or peptide fingerprint data, which, along with data from the gel and extensive proteomics databases, results in target identification. MALDI-TOF (Matrix-Assisted Laser Desorption Ionization - Time of Flight) mass spectrometry is often used for high throughput target identification.

For MALDI-TOF mass spectrometry, proteins must be spotted on a flat, metal MALDI target. This is a challenge for automated systems. HAMILTON's STAR line instruments provide maximum reliability and spot quality, due to our 0.1mm positional accuracy. In addition, the pressure-based liquid level detection system provides reliable pipetting of organic solvents. This method also eliminates the use of chromatographic tips for protein desalting, thereby reducing cost.

Protein spots produced by the STAR are well formed and accurately positioned on the MALDI target, and their diameter is consistently approx. 1mm. The 8-channel STAR workstation spots one 384 well target in approximately 2 hours. Using the 16-channel configuration, throughput can be increased to up to one 384-well target in 1 hour.

Features and
Benefits

  • Increased throughput, reliability and reduced costs
  • Up to 384 samples in 1 hour
  • 4 to 16 independently spreadable tips allow spotting for all MALDI targets available on the market
  • Accurate and reproducible spotting for 384- and 1536-spot targets
  • Automated z-height determination of the target plate

System
Configuration

  • MICROLAB STAR, 4-16 channels (depending on desired throughput)
  • Required labware and carriers for samples and reagents

Downloads

 
 
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