Target Validation

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Protein Crystallography
Assay Development - Statistical Parameter Optimization
Protein purification
Protein identification by MALDI Spotting, Gel Digest:

• In-Gel Protein Digestion
• MALDI Target Spotting

Lead Identification

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HTS – High throughput screening – no current applicationsSecondary screening:

• Enzymatic activity screening, serial dilutions
• Bacterial cell based assay
• ADME(T) – logP, logD, Kinetic Solubility, PAMPA, Protein Binding

Lead Optimization

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Thermodynamic solubility screening

Clinical Trials

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Individual/dynamic dilutions
ELISA Assays
Biomarker Screeningp>

Basic Research & Target Identification

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Genomic DNA Isolation
RNA Isolation
PCR set-up
Cloning
RNA interference
Reporter Genes
In-Gel Protein Digestion
MALDI Target Spotting

Compound Synthesis

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Combinatorial Chemistry
Compound Handling
Plate Reformatting
Hit picking

Pharmacokinetics

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Solid Phase extraction
Liquid/liquid extraction
LC/MS coupling
Protein Binding
ADME(T)–logP, logD, Kinetic Solubility, PAMPA, Protein Binding, CaCo2, Hepatocytes, Drug/Drug, Interaction, P450
Drug/Target Binding
Cell culture for cell-based screening

Drug Metabolism

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Hepatocytes
P450
RNA interference