For Micro-array Preparation
Oligonucleotide re-arraying
Micro-arraying has become a technique of choice for the study of gene expression. Re-Arrangement of even just a few hundred oligonucleotides by hand is not only tedious but highly prone to non-traceable errors. This is typically a task of choice for the MICROLAB® STAR because it requires precision pipetting and traceability, as well as flexibility. The MICROLAB® STAR can operate as part of a micro-array platform for spotting services for various micro-array applications, such as transcriptomics or genotyping for various model organisms.
Prior to micro-array spotting, libraries of oligonucleotides have to be rearranged and diluted according to a unique pattern to prepare 384-well microtiter plates ready to be used by the spotter. Dilution factors and patterns vary from one spotting project to another. These parameters can be entered into the system by a worklist. The high pipetting precision of the MICROLAB® STAR ensures repeatability in the dilutions.
Features and
Benefits
- Precision pipetting into 96- or 384-well plates
- High level of assay flexibility regarding number of plates and samples
- Flexible worklist handling and full traceability
- Flexible workstation configuration allowing processing of additional assays such as DNA purification, PCR set-up and DNA normalisation
System
Configuration
- MICROLAB® STAR, 8 channels
- Integrated robotic plate handler (iSWAP)
- Wash Station with 1 module for 300µl Steel Needles and 2 modules for 10µl Steel Needles
- BVS vacuum system
- Variomag shaker
- Temperature Controlled Carrier (TCC)
- BioRobotics MicroGrid II Pro spotter
- Ventana Discovery™ microarray hybridisation module
- Genetac LSIV scanner (Genomic Solutions)
- GenePix 4.O analysis software
Downloads
Links
www.genomicsolutions.com
(LSIV scanner & spotter)
www.axon.com
(analysis software)
www.ventanadiscovery.com
(micro-array hybridisation module)
